graphpad prism (7.0d) software  (Dotmatics Limited)

Journal: Journal of Medical Primatology

Article Title: CD 4 CD 8 Double Positive T cell responses during Mycobacterium tuberculosis infection in cynomolgus macaques

doi: 10.1111/jmp.12399

Figure Lengend Snippet: Frequency and function of CD 4 CD 8 T cells in PBMC and BAL during macaque Mycobacterium tuberculosis (Mtb) infection. A, Increased frequencies of CD 4 CD 8 T cells in PBMC s are seen at 4 wks after infection (from baseline) with an increase in Mtb‐specific production of TNF and granzyme B observed among CD 4 CD 8 T cells. Cytokine production in ESAT 6‐ CFP 10‐stimulated PBMC was subtracted from cytokine production by PBMC incubated with media. (B) An increased frequency of CD 4 CD 8 T cells from bronchoalveolar lavage ( BAL ) is observed soon after Mtb infection with increased Mtb‐specific TNF production. Samples were obtained before infection (“Pre”), at 4 (“4 wk”) and 12 (“12 wk”) weeks after Mtb infection, and at the time in which animals met the clinical definition of active or latent infection (“Dx”). Twenty‐four and 28 monkeys were included in the PBMC and BAL analyses, respectively. Kruskal‐Wallis tests with Dunn's multiple comparison post test were used to determine significance with P ‐values as indicated

Article Snippet: Kruskal‐Wallis test with Dunn's multiple comparison test was used to compare changes in cell type, cytokine production, and cytolytic marker expression over time within BAL and PBMC (GraphPad Prism 7.0d statistical software).

Techniques: Infection, Incubation, Comparison